Abcam, ab247725, Anti-Talin 2 antibody [EPR2536(2)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Talin 2 antibody. Carrier free. Suitable for WB and reacts with African green monkey, Rat, Mouse, Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR2536(2),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human, African green monkey,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab247725 is the carrier-free version of
ab108967
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Talin 2 also known as TLN2 is a large cytoskeletal protein approximately 270 kDa in mass. It acts mechanically as an important player in linking integrins to the actin cytoskeleton supporting the structural integrity of cells. Talin 2 is expressed in various tissues including muscle and neuronal systems with expression patterns similar to Talin 1 but has distinct roles in cellular adhesion and signal transduction.
Biological function summary
Talin 2 plays a significant role in cell adhesion and migration processes. It is part of a complex that includes integrins and other cytoskeletal proteins facilitating the transmission of mechanical signals across the cell membrane. This protein aids in focal adhesion assembly ensuring proper cellular responses to external stimuli. Talin 2 contributes to stabilizing intracellular interactions which are essential for maintaining tissue structure and cell motility.
Pathways
Talin 2 is deeply embedded in integrin-mediated signaling and the cytoskeletal organization pathway. It modulates interactions between integrins and F-actin orchestrating actin dynamics and contributing to cellular adherence junctions. Talin 2 functions closely with proteins like vinculin which supports actin filament linkage and reinforces mechanical stability under tension.
Talin 2 has been linked to muscular dystrophies and certain neuropathies. Mutations or dysregulation in Talin 2 can impair muscle function as its interaction with dystrophin is critical for muscle integrity. Additionally disruptions in Talin 2 mediated signaling could contribute to neurodegenerative disorders where its interactions with integrins can affect neuronal connectivity and survival. Understanding Talin 2's role in these conditions may provide insights for therapeutic interventions.