Abcam, ab247823, Anti-Ube2N / Ubc13 antibody [EPR5163] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Ube2N / Ubc13 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5163,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab247823 is the carrier-free version of
ab109294
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please
contact us
for more information.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ube2N also known as Ubc13 is a ubiquitin-conjugating enzyme with a molecular mass of around 17 kDa. It plays an important role in the ubiquitination process by forming a complex with Uev1A which assists in catalyzing the attachment of ubiquitin to substrate proteins. Expression of Ube2N is found in various tissues particularly in those with high rates of protein modification and signal regulation. Mechanically Ube2N works by forming polyubiquitin chains linked through lysine 63 on ubiquitin molecules a step essential for signaling rather than protein degradation.
Biological function summary
Ube2N has a significant role in DNA repair and immune response. It teams up with other proteins as part of larger complexes like the E2/E3 ligase complexes facilitating non-degradative polyubiquitin chains that activate signaling pathways. These chains contribute to the repair of DNA double-strand breaks by involving in the activation of DNA repair proteins and maintaining genomic integrity. Furthermore Ube2N is pivotal in modulating the immune response by activating NF-kB a transcription factor important for immune and inflammatory responses.
Pathways
Ube2N's activities impact the important signaling pathways such as NF-kB and DNA damage response pathways. In the NF-kB pathway K63-linked polyubiquitination by Ube2N leads to the activation of IKK complex subsequently mobilizing NF-kB to the nucleus to initiate transcription of immune genes. In DNA damage response Ube2N interacts with proteins like Ubc13 and Rnf8 to enhance DNA repair processes. These interactions highlight Ube2N's connection within a network of proteins maintaining cell health.
Ube2N is linked to cancer progression and immune system dysfunctions. Abnormal activity or expression of Ube2N can lead to unchecked cellular proliferation seen in various types of cancer. Additionally Ube2N associates with immune disorders where deregulated NF-kB activity contributes to chronic inflammation and auto-immune conditions. Its interaction partners like Uev1A can also play a role in the dysregulated pathways contributing to these diseases.