Abcam, ab247919, Anti-TORC1 antibody [EPR3381(2)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal TORC1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR3381(2),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab247919 is the carrier-free version of
ab109736
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TORC1 also known as Target of Rapamycin Complex 1 is a protein complex that regulates cell growth and metabolism in response to nutrients and growth factors. This complex contains proteins such as mTOR Raptor and mLST8. The mass of mTOR the catalytic subunit is approximately 289 kDa. TORC1 is mostly expressed in tissues with high metabolic activity including the liver muscle and adipose tissue. It functions as a serine/threonine kinase playing a significant role in protein synthesis autophagy and energy metabolism.
Biological function summary
TORC1 holds a central role in cellular responses to nutrients and energy status. It is a part of the larger mechanistic target of rapamycin (mTOR) signaling pathway. This pathway influences essential cellular processes such as growth proliferation and survival. TORC1 activity increases in the presence of nutrients such as amino acids and is necessary for ribosomal biogenesis and translation initiation. It also acts as an important regulator of autophagy where its inhibition promotes autophagic degradation of cellular components balancing energy and resource availability.
Pathways
TORC1 integrates signals from related pathways such as PI3K-AKT and AMPK. These pathways facilitate the balance between anabolic and catabolic processes. The PI3K-AKT pathway stimulates TORC1 activity promoting cell growth and proliferation while the AMPK pathway inhibits TORC1 during low energy states conserving cellular resources. Proteins like TSC1/TSC2 complex and Rheb interact with TORC1 modulating its kinase activity based on cellular and environmental conditions.
TORC1 dysregulation links to conditions like cancer and type 2 diabetes. Overactivation of TORC1 contributes to unchecked cell proliferation in various cancers. Agents that inhibit TORC1 activity such as rapamycin show potential in cancer treatment to curb tumor growth. In type 2 diabetes TORC1 is connected through insulin signaling pathways as excess TORC1 activity may lead to insulin resistance. Proteins like IRS-1 which interacts with TORC1 play a role in these metabolic and proliferative disorders.