Abcam, ab247966, Anti-LHX3/LIM antibody [EPR3344(2)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal LHX3/LIM antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR3344(2),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab247966 is the carrier-free version of
ab124697
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LHX3 also known as LIM homeobox 3 protein is a transcription factor involved in the development and differentiation of specific tissues in the human body. It is expressed mainly in the pituitary gland forebrain and spinal cord. The molecular weight of LHX3 is approximately 45 kDa. This transcription factor contains two LIM domains and one homeodomain which are significant for its DNA-binding capability. Through these domains LHX3 regulates the expression of target genes critical for tissue-specific development.
Biological function summary
LHX3 is essential for the proper formation of the pituitary gland and motor neurons. It acts as part of a complex that includes other LIM homeodomain proteins to modulate transcription of genes responsible for lineage specification. LHX3 functions by binding to DNA sequences and affecting the transcriptional activation or repression of gene targets influencing processes like hormone biosynthesis in the pituitary. This regulation ensures appropriate cellular responses during development and differentiation.
Pathways
LHX3 interacts within the Wnt and Notch signaling pathways which are pivotal in developmental processes. In the Wnt pathway it modulates gene expression that governs cell proliferation and differentiation. The Notch pathway involvement highlights its role in neural differentiation. Through these pathways LHX3 works closely with proteins like β-catenin in the Wnt pathway impacting how cells communicate and respond to developmental cues.
LHX3 mutations are associated with Combined Pituitary Hormone Deficiency (CPHD) and pituitary hormone synthesis issues resulting in growth and developmental disorders. LHX3 defects can interact with other proteins like PIT1 influencing the clinical presentation of CPHD. The understanding of LHX3's role in these pathways and its interaction with proteins offers insight into therapeutic approaches to mitigate these disorders.