Abcam, ab248020, Anti-Cdc25B antibody [EPR3459(2)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Cdc25B antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR3459(2),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248020 is the carrier-free version of
ab124819
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Cdc25B phosphatase also known as M-phase inducer phosphatase 2 or CDC25B2 plays an important role in cell cycle regulation by activating cyclin-dependent kinases (CDKs). It weighs approximately 62 kDa. Researchers observe its expression in normal and cancerous tissues with higher levels found in proliferating cells. Cdc25B localizes to the nucleus during the cell cycle where it exerts control over cell division by removing inhibitory phosphates on CDKs.
Biological function summary
This protein accelerates the transition through various phases of the cell cycle. It does not function alone but forms part of a complex regulatory network ensuring timely CDK activation. By promoting the G1/S and G2/M transitions Cdc25B enables cells to progress through the cell cycle. Additionally Cdc25B is involved in DNA damage checkpoints helping cells respond to genotoxic stress thereby ensuring genomic stability through complex regulatory interactions.
Pathways
Several key cell cycle pathways include Cdc25B. It acts prominently in the cell cycle checkpoint control pathway and the DNA damage response pathway. The protein directly activates CDC2/CDK1 through dephosphorylation contributing to cell cycle progression. Cdc25B interacts with regulatory proteins like ATM/ATR which modulate Cdc25B activity in response to DNA damage further integrating it into critical checkpoint pathways.
Researchers link Cdc25B to cancer and neurological disorders. Overexpression of Cdc25B often associates with various cancers where it may contribute to unchecked cell proliferation through its influence on CDK activity. In this context it connects with oncogenic proteins like Cyclin B1 providing potential targets for therapeutic intervention. Additionally malfunction in Cdc25B activity associates with neurodegenerative diseases due to its role in cell cycle regulation and DNA repair mechanisms. Scientists are exploring its relationship with proteins such as p53 in these diseases offering insights into potential treatment strategies.