Abcam, ab248275, Anti-beta 1 Spectrin antibody [EPR6259] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal beta 1 Spectrin antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR6259,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248275 is the carrier-free version of
ab129065
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Beta 1 Spectrin also known as SPTB is a large cytoskeletal protein with a mass of approximately 247 kDa. It mechanically contributes to the stabilization of the plasma membrane by forming a meshwork beneath it. This protein links the plasma membrane to the actin cytoskeleton. SPTB is abundantly expressed in erythrocytes and neurons where it plays important roles in maintaining cell shape and structural integrity.
Biological function summary
Beta 1 Spectrin is part of the spectrin-actin cytoskeleton complex and participates in cellular functions such as maintaining membrane integrity and flexibility. It anchors various proteins to the cytoskeleton and regulates membrane protein distribution. The interactions of beta 1 Spectrin within cells ensure proper localization of membrane channels and receptors critical for cellular signaling and transport activities.
Pathways
Beta 1 Spectrin functions in pathways connected to membrane cytoskeleton dynamics and cellular environmental responses. It closely relates to the ankyrin pathway where it interacts with ankyrin proteins to connect spectrin-actin network to plasma membrane proteins. Furthermore beta 1 Spectrin is involved in the maintenance and organization of lipid rafts which are specialized membrane microdomains enriched with specific lipids and proteins.
Beta 1 Spectrin mutations and defects associate with conditions like hereditary spherocytosis and spinocerebellar ataxia. In hereditary spherocytosis defects in beta 1 Spectrin lead to hemolytic anemia due to the altered erythrocyte membrane stability. This protein also interacts with ankyrin and band 3 proteins which are important in red blood cell membrane architecture and function. In spinocerebellar ataxia abnormalities in beta 1 Spectrin affect neuronal function contributing to progressive motor coordination issues.