Abcam, ab248342, Anti-PGAM1 antibody [EPR8042] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal PGAM1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR8042,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248342 is the carrier-free version of
ab129191
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Phosphoglycerate mutase 1 (PGAM1) also known as PGAM-M or brain PGAM is an enzyme with a molecular weight of approximately 29 kDa. PGAM1 catalyzes the conversion of 3-phosphoglycerate to 2-phosphoglycerate in the glycolytic pathway. This enzyme is expressed in a wide range of tissues with significant levels found in brain and muscle cells. It plays an important role in energy metabolism by facilitating the intermediate steps of glycolysis.
Biological function summary
PGAM1 acts within the glycolysis and gluconeogenesis processes interacting with other metabolic enzymes to maintain cellular energy balance. It functions as a part of the phosphoglycerate mutase enzyme complex. The activity of PGAM1 is important for optimal glycolytic flux directly linking it to energy production and regulation within the cell. By maintaining the concentrations of glycolytic intermediates PGAM1 supports various cellular processes that depend on energy generated through glycolysis.
Pathways
The activity of PGAM1 integrates glycolysis with other vital metabolic processes in the cell. One key pathway involving PGAM1 is the glycolytic pathway where it collaborates with the enzymes phosphoglycerate kinase and enolase to efficiently drive the conversion of glucose to pyruvate. Additionally PGAM1 is linked to the pentose phosphate pathway where it indirectly influences nucleotide biosynthesis and antioxidant responses by affecting the flux of metabolites into the pathway.
PGAM1's involvement in energy metabolism connects it to various cancer types. Many cancer cells display elevated levels of PGAM1 which can promote enhanced aerobic glycolysis also known as the Warburg effect. This shift in metabolism is associated with increased cancer cell proliferation and survival. Furthermore PGAM1's interaction with other proteins like p53 a tumor suppressor highlights its potential role in tumorigenesis where dysregulation of these interactions can contribute to cancer progression.