Abcam, ab248408, Anti-BAF57/SMARCE1 antibody [EPR8848] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal BAF57/SMARCE1 antibody. Carrier free. Suitable for ChIP, WB, IHC-P, ICC/IF, ChIC/CUT&RUN-seq and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR8848,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, ChIC/CUT&RUN-seq, ICC/IF, ChIP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248408 is the carrier-free version of
ab131328
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
BAF57 also known as SMARCE1 is a component of the SWI/SNF chromatin-remodeling complex. It aids in regulating the structure of chromatin influencing gene expression. BAF57 weighs approximately 53 kDa and is expressed widely across various tissue types with notable presence in the brain and reproductive organs. This protein plays an important role in reshaping chromatin making it essential for modulating the accessibility of transcription factors to DNA.
Biological function summary
As a part of the SWI/SNF complex BAF57/SMARCE1 facilitates the remodeling of chromatin impacting transcription regulation. By interacting directly with DNA BAF57 allows chromatin sliding and repositioning therefore influencing gene transcription. It plays a significant role in embryonic development neural progenitor cell proliferation and the regulation of cell cycle and differentiation processes. The protein helps modulate responses to hormonal and growth factor signals which are integral for proper cellular control.
Pathways
BAF57/SMARCE1 functions mainly within the WNT signaling and the nuclear hormone receptor pathways. It initiates changes in chromatin architecture impacting gene expression in pathways including those controlling cellular differentiation and development. Proteins like SMARCA4 and ARID1A often work alongside BAF57 in these pathways helping to modulate signal transduction processes that are important for cellular outcomes.
BAF57/SMARCE1 has links to certain cancers and Coffin-Siris syndrome. Mutations in this protein or alterations in its expression can disrupt normal SWI/SNF complex function leading to abnormal cell growth and division contributing to tumor development. Additionally genetic deficits in BAF57 have associated with neural development disorders particularly through the altered function of related proteins like SMARCB1 and SMARCA4 further elucidating its role in various pathological conditions.