Abcam, ab248454, Anti-alpha 2 Macroglobulin antibody [EPR4431] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal alpha 2 Macroglobulin antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR4431,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248454 is the carrier-free version of
ab133299
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alpha-2-macroglobulin also known as alpha-2-M and A2M is a large glycoprotein with a molecular mass of approximately 725 kDa. It is expressed in the liver and is found in high concentrations in the blood plasma. This protein functions mechanically as a broad-spectrum protease inhibitor. It can trap proteases within its structure preventing them from accessing substrates. Additionally alpha-2-macroglobulin can bind to cytokines and growth factors altering their availability and activities.
Biological function summary
Alpha-2-macroglobulin plays an important role in maintaining homeostasis by regulating protease activities and modulating immune system reactions. It does not form a permanent complex but can transiently associate with the proteases and various molecules mentioned. By interacting with proteases it controls processes like coagulation and fibrinolysis and participates in the clearance of proteinases and growth factors from the circulation. It influences cell signaling pathways and inflammatory responses highlighting its significance in maintaining balance within the body.
Pathways
Alpha-2-macroglobulin is an important component in the immune response and coagulation pathways. In the complement system it binds with complement components to regulate their activities. It associates with proteins such as plasmin and thrombin which are important for blood clotting and fibrinolysis. These relationships emphasize the protein's involvement in both protective mechanisms against pathogens and the regulation of blood coagulation and breakdown connecting the immune and hemostatic systems.
Alpha-2-macroglobulin has been linked to Alzheimer's disease and liver diseases. In Alzheimer's altered levels of this protein might affect the amyloid-beta peptide clearance due to its protease inhibition and binding properties. It associates with proteins like lipoprotein receptor-related protein-1 (LRP1) influencing amyloid-beta metabolism. In liver diseases its levels can indicate liver dysfunction or inflammation given its synthesis in the liver and can relate to proteins involved in inflammation such as cytokines. Its modulation of protease activity makes it a critical target in understanding these disorders.