Abcam, ab248515, Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Ube1L antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR4269(2),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:Flow Cyt (Intra), WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248515 is the carrier-free version of
ab133479
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ube1L also known as E1 Ubiquitin Activating Enzyme 1 or UBA1 is a protein that plays a critical role mechanically in the ubiquitination process. This process involves the tagging of proteins for degradation by the proteasome which is essential for regulating protein turnover in cells. Ube1L has an approximate mass of 117 kDa and is expressed in various tissues across the body including the liver and muscle. The enzyme catalyzes the first step in the ubiquitination pathway by activating ubiquitin molecules in an ATP-dependent manner which is necessary for transferring ubiquitin to substrate proteins.
Biological function summary
Ube1L is essential for maintaining protein homeostasis within the cell by regulating the degradation of misfolded damaged or unneeded proteins. It operates as part of the ubiquitin-proteasome system a complex assembly of various enzymes and proteins that coordinate the target-specific degradation of proteins. This ubiquitin-proteasome system helps control protein levels to ensure cellular functions such as DNA repair cell cycle progression and apoptosis occur smoothly.
Pathways
Ube1L is integral in the ubiquitin-dependent catabolic pathway and the NF-κB signaling pathway. In the ubiquitin-dependent catabolic pathway Ube1L works alongside proteins like UbcH5B and Ubc13 to transfer ubiquitin to substrates that are marked for destruction. Then in the NF-κB signaling pathway Ube1L indirectly regulates transcription factors which influences immune response and inflammation through the conjugation and deconjugation of ubiquitin on inhibitor proteins like IκB.
Disruptions in Ube1L function associate with cancer and neurodegenerative disorders such as amyotrophic lateral sclerosis (ALS). Cancer cells often exploit the dysregulation of the ubiquitin-proteasome system to avoid apoptosis and grow uncontrollably. Ube1L’s interaction with proteins like p53 a tumor suppressor highlights its role in cancer pathogenesis. In ALS abnormal protein accumulations that result from inefficient ubiquitination link back to Ube1L's function and its interaction with proteins like TDP-43 has been studied to understand its contribution to disease progression.