Abcam, ab248599, Anti-MC-2 antibody [EPR8386] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal MC-2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR8386,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248599 is the carrier-free version of
ab133656
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MC2 also known as adrenocorticotropic hormone receptor (ACTHR) plays a critical role in the adrenal gland's function by binding ACTH. This receptor is a G protein-coupled receptor with a mass of approximately 33 kDa. MC2 expression is localized mainly in the adrenal cortex where it facilitates the regulation of adrenal hormone production essential for physiological stress response.
Biological function summary
The adrenocorticotropic hormone receptor mediates the effects of ACTH by stimulating adrenal steroidogenesis. It serves as part of the MC2 receptor complex which includes the MC2 receptor accessory protein (MRAP). This complex is key in MC2's activation and proper targeting to the cell surface important for cortisol production in response to stress stimuli.
Pathways
MC2 is significantly involved in the hypothalamic-pituitary-adrenal (HPA) axis. This pathway regulates stress-induced hormone release where MC2 interacts with proteins such as MRAP to mediate cortisol secretion. The action of MC2 influences the downstream signaling pathways including the cAMP/PKA pathway which plays a pivotal role in hormone synthesis and secretion regulation.
Alterations in MC2 function are linked to conditions such as familial glucocorticoid deficiency type 1 (FGD1) and Cushing's syndrome. Familial glucocorticoid deficiency arises due to MC2 mutations affecting adrenal response leading to insufficient cortisol levels. The receptor's function interplays with MRAP essential for disease manifestation and a potential therapeutic target. Meanwhile Cushing's syndrome characterized by excessive cortisol may involve MC2 dysregulation in adrenal hyperplasia-related mechanisms.