Abcam, ab248620, Anti-RRM1 antibody [EPR8482] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal RRM1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR8482,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248620 is the carrier-free version of
ab133690
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RRM1 also known as ribonucleotide reductase large subunit or RNR1 plays an important role in DNA synthesis and repair converting ribonucleotides to deoxyribonucleotides. This enzyme has a mass of approximately 90 kDa. RRM1 is found in multiple tissues exhibiting especially high expression levels in proliferative tissues and tumors. Its activity is tightly regulated through the cell cycle and it is essential during DNA replication and cell division.
Biological function summary
The large subunit of ribonucleotide reductase contributes to the enzymatic function through binding and activation of smaller subunits including RRM2 and RRM2B forming a complex. This complex is important for maintaining balanced deoxyribonucleotide pools during the S-phase of the cell cycle. Proper RRM1 function ensures the fidelity of DNA synthesis impacting genomic stability and cell viability.
Pathways
RRM1 acts within the DNA replication and repair pathways. It interacts with the tumor suppressor protein p53 which modulates its activities in response to DNA damage. Through pathways like the p53-dependent checkpoint RRM1 contributes to DNA damage response mechanisms. This interaction with p53 and involvement in the DNA replication pathway highlight its centrality in maintaining genomic integrity.
RRM1 has connections with cancer and chemoresistance. Overexpression of RRM1 often correlates with increased tumor aggressiveness and poor prognosis in several cancers including lung and pancreatic cancers. In the context of chemoresistance RRM1 interplays with the excision repair cross-complementation group 1 protein (ERCC1) impacting the effectiveness of certain chemotherapies. Its role in these conditions makes it a target for cancer diagnosis and treatment strategies.