Abcam, ab248638, Anti-Syntaxin 3 antibody [EPR8543] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Syntaxin 3 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR8543,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248638 is the carrier-free version of
ab133750
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Syntaxin 3 sometimes known as Stx3 is a protein involved in vesicle trafficking and membrane fusion. It plays a mechanical role by facilitating the fusion of transport vesicles with target membranes an essential step in intracellular transport mechanisms. Syntaxin 3 is a member of the t-SNARE family and has a molecular mass of approximately 33 kDa. This protein expresses mainly in epithelial cells including those in the kidney and lungs as well as certain neuronal tissues indicating its involvement in critical cellular processes in these areas.
Biological function summary
Syntaxin 3 acts in cellular transport by participating in the trans-Golgi network-to-plasma membrane pathway. This protein is part of the SNARE complex a group of proteins that mediates vesicle fusion. By engaging with other SNARE proteins like SNAP-25 and VAMP Syntaxin 3 helps ensure the precise delivery of vesicle contents to the plasma membrane. Its role is important for maintaining cellular homeostasis by regulating how cells communicate and exchange materials with their environment.
Pathways
Syntaxin 3 is an integral component of the exocytosis pathway and is involved in the secretory pathway. In exocytosis Syntaxin 3 associates with proteins such as Munc18 and synaptotagmin which help in vesicle docking and fusion. In the secretory pathway Syntaxin 3 is responsible for directing proteins and lipids to their intended membrane sites thereby influencing cellular communication. These interactions guide the flow of proteins within vesicle transport networks and coordinate various signaling events within the cell.
Syntaxin 3 has connections to conditions such as cystic fibrosis and certain types of diabetes. In cystic fibrosis abnormal function of Syntaxin 3 may disrupt normal secretory processes in epithelial cells contributing to enhanced pathology. The protein is associated with the cystic fibrosis transmembrane conductance regulator (CFTR) protein which fails to localize correctly in this disease. In diabetes Syntaxin 3 impacts insulin secretion due to its role in pancreatic beta cells tying it to the disorder through insulin-regulating mechanisms with proteins such as GLUT4.