Abcam, ab248678, Anti-EDD antibody [EPR4841] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal EDD antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR4841,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248678 is the carrier-free version of
ab134089
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please
contact us
for more information.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EDD also known as EDD1 or EDDC is a significant target within cellular processes. This protein possesses a molecular mass of approximately 300 kDa and functions as a ubiquitin-protein ligase. EDD is highly expressed in many tissues with noticeable levels in reproductive tissues brain and pancreas. It plays a mechanical role in tagging proteins for degradation via the ubiquitin-proteasome pathway contributing to protein turnover and regulation.
Biological function summary
EDD or EDDC acts as a central regulator of cell cycle progression and DNA damage response. It is part of the complex known as EDD-BA where it interacts with other proteins to control cellular processes. One main function of EDD lies in mediating the degradation of proteins preventing the accumulation of destructive or malfunctioning proteins that could harm cell integrity. Through its ligase activity EDD modifies target proteins with ubiquitin guiding them toward their respective cellular fates.
Pathways
EDD interacts with several essential cellular pathways including the cell cycle and the TGF-beta signaling pathway. In the cell cycle pathway EDD collaborates with proteins like p53 to ensure proper cell cycle arrest and DNA repair when damage is detected. The involvement of EDD in the TGF-beta pathway highlights its role in cellular growth and apoptosis. Through these interactions EDD maintains cellular order and helps respond to cellular stress and damage.
EDD has associations with cancer and developmental disorders. Abnormalities in EDD expression or function can lead to uncontrolled cell proliferation linking it to various cancers. Its interaction with p53 reinforces this connection as p53 mutations are common in cancers. Furthermore mutations in EDD have been implicated in developmental disorders where disrupted protein regulation impacts cellular growth and differentiation processes. Understanding EDD's role in these contexts provides insights into therapeutic targets and disease mechanisms.