Abcam, ab248717, Anti-NOP10 antibody [EPR8857] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal NOP10 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR8857,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248717 is the carrier-free version of
ab134902
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NOP10 also known as Nucleolar Protein 10 is a small protein component with a molecular weight of approximately 7 kDa. It is an essential part of the H/ACA ribonucleoprotein complexes responsible for guiding the isomerization of uridine to pseudouridine in ribosomal RNA (rRNA). NOP10 is expressed in the nucleolus where it interacts closely with other core proteins such as NHP2 NOP58 and the catalytic subunit dyskerin also known as DKC1.
Biological function summary
NOP10 plays a critical role in ribosome biogenesis by taking part in H/ACA snoRNP complexes that facilitate the processing and modification of rRNA which is necessary for proper ribosome function. The protein forms a complex with the other core proteins and small nucleolar RNAs (snoRNAs) essential for the chemical alteration and folding of rRNA. By supporting the formation and stability of these complexes NOP10 ensures the fidelity of ribosomal assembly and cellular protein synthesis.
Pathways
The protein contributes significantly to the ribosome biogenesis and RNA modification pathways. It directly influences the ribosome biogenesis process which determines the production of ribosomes essential for protein translation in cells. In this pathway NOP10 interacts with proteins such as GAR1 and dyskerin which assist in assembling the complete ribosomal subunits. These interactions help sustain the delicate balance of rRNA modifications required for functional ribosome activity during protein synthesis.
NOP10 is associated with conditions like dyskeratosis congenita and some forms of cancer. Dyskeratosis congenita a genetic disorder characterized by bone marrow failure and skin abnormalities is linked to mutations in components of the H/ACA snoRNP complex including NOP10. In cancer altered expression or functionality of NOP10 can influence the process of ribosome biogenesis leading to increased cellular proliferation. Dyskerin another key member of the complex is also implicated in these conditions highlighting the intertwined role of these proteins in maintaining cellular homeostasis and their potential disruption in disease states.