Abcam, ab248877, Anti-Semenogelin I antibody [EPR3675(2)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Semenogelin I antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR3675(2),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248877 is the carrier-free version of
ab139405
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Semenogelin I sometimes abbreviated as SgI acts mechanically as an important component in the formation of the semen coagulum. This protein has a molecular mass of approximately 52 kDa and is expressed chiefly in the seminal vesicles. After ejaculation Semenogelin I contributes significantly to the formation of a gel-like matrix which plays an important role in the initial viscosity of seminal fluid. Upon the cleavage of Semenogelin I by prostate-specific antigen (PSA) this gel liquefies allowing sperm to achieve motility.
Biological function summary
The primary role of Semenogelin I lies in its involvement in semen coagulation and liquefaction processes. It forms a complex with other seminal plasma proteins to stabilize the gel structure. This structural role ensures that Semenogelin I helps regulate the controlled release and proper function of spermatozoa. Additionally it is implicated in modulating immune system responses against male seminal antigens to prevent adverse reactions in the female reproductive tract.
Pathways
The actions of Semenogelin I integrate it into reproductive and coagulation pathways. In the context of semen coagulation and liquefaction it works closely with PSA which breaks down Semenogelin I and facilitates liquefaction and sperm motility. This process ties Semenogelin I into the prostaglandin signaling pathway which affects reproductive physiology and fertility. Furthermore proteins such as fibronectin and lactoferrin relate to Semenogelin I within these pathways assisting in post-ejaculation changes that enhance sperm function and fertility.
Semenogelin I is linked to fertility issues and prostate cancer. Abnormal levels or altered cleavage patterns can contribute to male infertility potentially through improper semen liquefaction that affects sperm motility. In prostate cancer elevated degradation of Semenogelin I by enzymes like PSA could serve as an indicator of tumor activity and progression. Consequently monitoring Semenogelin I alongside PSA levels can offer insights into both male reproductive health and prostate cancer management.