Abcam, ab248932, Anti-Tat-SF1 antibody [EPR9104(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal HTSF1 antibody. Carrier free. Suitable for WB, ICC/IF, IP, IHC-P and reacts with Human, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR9104(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:ICC/IF, WB, IHC-P, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248932 is the carrier-free version of
ab140654
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please
contact us
for more information.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Tat-SF1 also known as transcription elongation factor SPT5 (SPT4-5) is a protein integral to the RNA transcription process. The mass of Tat-SF1 is approximately 90 kDa. It is primarily expressed in the nucleus of the cell where it interacts with RNA Polymerase II to facilitate the elongation phase of transcription. Tat-SF1 plays a significant role by enhancing the transcriptional speed and efficiency critical for the production of various RNA species.
Biological function summary
Tat-SF1 functions by participating in the regulation of transcription elongation. It is part of the larger multi-protein complex involving CycT1 another important transcription factor. This complex helps modulate the interaction of RNA polymerase II with other transcriptional machinery and influences the synthesis of mRNA. Through these actions Tat-SF1 supports the accurate gene expression important for normal cellular processes and response to environmental cues.
Pathways
Tat-SF1 has functions within the general transcriptional regulation pathway and the HIV-1 viral transcription pathway. It directly interacts with the HIV-1 Tat protein; this interaction is essential for efficient transcription of HIV-1 genes. The connection with the CycT1 protein is important within these pathways as it aids in recruiting Tat-SF1 to the sites where RNA polymerase II needs support therefore coordinating a precise transcriptional response.
Tat-SF1 is notably linked to HIV-1 infection due to its interaction with the viral Tat protein facilitating viral replication. Disruption or alteration in the function of Tat-SF1 can hinder this process making it a target for therapeutic interventions. Additionally any dysregulation in the normal transcriptional process involving Tat-SF1 and CycT1 can contribute to oncogenesis as improper gene expression can lead to unregulated cell proliferation. Understanding these connections highlights the importance of Tat-SF1 in disease mechanisms and therapeutic strategy development.