Abcam, ab249024, Anti-TMF antibody [EPR10671(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal TMF antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR10671(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, ICC/IF, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249024 is the carrier-free version of
ab151702
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Transcriptional mediator factor (TMF) also known as TMF/ARA160 weighs around 160 kDa. It resides in the nucleus and cytoplasm of cells. TMF is a multifunctional protein and plays a part in transcription regulation. It interacts with protein complexes influencing their stability and function. In addition to its nuclear roles TMF associates with the Golgi apparatus suggesting its diverse function in different cellular contexts.
Biological function summary
The role of TMF extends to various cellular processes including transcriptional regulation signal transduction and vesicle transport. It forms part of larger protein complexes which modulate gene expression and cellular signaling networks. TMF serves as a scaffold facilitating the interactions between transcription factors and other regulatory proteins. This makes TMF an active regulator of transcriptional control impacting gene expression patterns.
Pathways
Many known cellular pathways require TMF's involvement. TMF impacts the NF-kappaB signaling pathway and the Wnt signaling pathway. It directly interacts with proteins like RelA in the NF-kappaB pathway where it modulates transcriptional activation. TMF's influence on these pathways highlights its significant role in regulating cellular responses and maintaining cellular homeostasis.
Research shows that TMF has associations with cancer and neurodegenerative diseases. Its abnormal expression or mutation links to tumorigenesis particularly in cancers such as prostate and breast cancer. Its interaction with proteins such as beta-catenin in the Wnt pathway associates TMF with oncogenic processes. Moreover TMF's role in the Golgi apparatus links it to neurodegenerative disorders where disruptions in vesicle transport are common features.