Abcam, ab249047, Anti-Smg1 antibody [EPR4591(2)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal SMG1 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR4591(2),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249047 is the carrier-free version of
ab151730
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Smg1 also known as SMG-1 is a large protein kinase with a molecular mass of approximately 410 kDa. It localizes mainly in the cytoplasm and nucleus of cells and has an important mechanical role in phosphorylating proteins to regulate various cellular processes. As a part of the phosphatidylinositol 3-kinase-related kinase (PIKK) family Smg1 exhibits enzymatic activity that is important for maintaining cellular functions.
Biological function summary
Smg1 plays an important part in the nonsense-mediated mRNA decay (NMD) pathway an important cellular process for RNA surveillance mechanisms. Smg1 associates with other proteins forming a complex known as the SMG1C complex which monitors and degrades faulty mRNAs that contain premature stop codons to prevent the production of truncated proteins. Smg1 aids in safeguarding the cellular transcriptome integrity by ensuring quality control at the mRNA level.
Pathways
Smg1 interacts importantly within the NMD pathway alongside proteins UPF1 and UPF2 which are essential for proper function. Smg1 is also involved in the cellular response to stress pathways contributing to the regulation of stress granules. Through these pathways Smg1 ensures proper adaptation and response to changing cellular conditions maintaining overall cell health and functionality.
Smg1 has implications in cancer and genetic disorders. Aberrations in Smg1 activity or expression can lead to improper mRNA decay contributing to tumorigenesis by allowing defective mRNAs to produce malfunctioning proteins. In cancer Smg1 regulates p53 a protein that is associated with cell cycle control and apoptosis. Additionally mutations affecting Smg1 can relate to neurodevelopmental disorders where it influences proteins involved in mRNA processing pathways highlighting its role in nervous system function and development.