Abcam, ab249049, Anti-Cytohesin 1 antibody [EPR10051] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Cytohesin 1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR10051,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249049 is the carrier-free version of
ab151732
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cytohesin 1 also known as CYTH1 or PSCD1 belongs to the cytohesin family. It plays a role as a guanine nucleotide exchange factor (GEF) specifically for Arf (ADP-ribosylation factor) proteins. Cytohesin 1 weighs approximately 47 kDa. It contributes to cellular communication and membrane trafficking activities by activating Arf family GTPases. You can find it widely expressed in tissues including immune cells and the nervous system. It localizes to cytoplasmic regions and associates with the plasma membrane through a pleckstrin homology (PH) domain.
Biological function summary
Cytohesin 1 influences multiple cellular processes like actin cytoskeleton remodeling cell migration and adhesion. It directs signal transduction pathways that regulate these key processes contributing to how cells interact with their external environment. Although Cytohesin 1 can function alone it typically operates as part of protein complexes that include partners like ARNO (another GEF for Arf proteins) that fine-tune intracellular signaling dynamics. This protein also interacts with other signaling molecules through its Sec7 and PH domains expanding its role across various cellular contexts.
Pathways
Cytohesin 1 integrates into significant signaling cascades such as the Arf6 pathway and the phosphoinositide 3-kinase (PI3K) pathway. Through these pathways it compounds cellular responses related to vesicular trafficking and cell morphology adjustments. In the Arf6 pathway Cytohesin 1 acts alongside proteins such as Arf6 and EFA6 to modulate membrane dynamics essential for endocytosis and stress fiber formation. In the PI3K pathway it collaborates with signaling molecules to regulate growth factor-mediated responses highlighting the interconnectivity Cytohesin 1 maintains between various pathway components.
Cytohesin 1 has been linked to immune-related diseases and neurological disorders. In the context of the immune system it plays a role in T cell activation and inflammation and alterations in its functioning may contribute to conditions like autoimmunity. In neurological contexts Cytohesin 1 interacts with proteins related to cellular adhesion and neurite outgrowth which can impact neurological functions and may play a role in developmental disorders. Its relationship with proteins like integrins also suggests how its dysregulation can affect cell attachment and signaling impacting disease progression.