Abcam, ab249086, Anti-KBTBD8 antibody [EPR10698(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal KBTBD8 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR10698(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249086 is the carrier-free version of
ab154189
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
KBTBD8 also known as Kelch repeat and BTB domain-containing protein 8 serves a mechanical role in the stabilization and ubiquitination processes. It functions as an adapter protein connecting specific substrates to a core ubiquitin ligase complex facilitating substrate degradation through the proteasome. KBTBD8 has an approximate mass of 69 kDa. In terms of expression KBTBD8 is found in various tissues with significant levels observed in the brain and developing tissues indicating its importance in developmental processes.
Biological function summary
KBTBD8 acts in cellular regulation and developmental processes. It is involved in the formation of a complex that mediates specific substrate recognition essential for precise protein turnover and cellular homeostasis. Evidence shows its role in neural tube development by regulating cytoskeletal dynamics emphasizing its contribution to embryonic morphogenesis. KBTBD8 activity requires interaction with other molecules in the ubiquitination pathway ensuring regulated proteolysis necessary for normal cellular function.
Pathways
The involvement of KBTBD8 extends into the ubiquitin-proteasome pathway and cytoskeletal regulation pathways. An important pathway includes its association with CRL4 E3 ligase complex a critical component that modulates cell cycle progression and response to DNA damage. Through these pathways KBTBD8 interacts with proteins such as CUL4 and DDB1 which play essential roles in maintaining genomic stability and cellular integrity.
KBTBD8 relates to conditions like neural tube defects and certain cancers. Its abnormal expression or malfunction may contribute to improper embryonic development manifesting as neural tube defects. Furthermore disrupted regulation of the ubiquitination pathway involving KBTBD8 can lead to oncogenesis with proteins such as p53 being affected through altered ubiquitin-mediated processes. These associations highlight the significance of KBTBD8 in maintaining cellular and developmental equilibrium.