Abcam, ab249120, Anti-S31 antibody [EPR10789(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal S31 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR10789(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249120 is the carrier-free version of
ab154813
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The S31 protein also known as the Influenza A virus proton channel M2 facilitates the pH regulation during viral infection. It functions as a transmembrane ion channel and plays an essential role in the viral uncoating process. S31 is a relatively small protein with a mass of roughly 97 amino acids. It is primarily expressed in the membranes of infected host cells during the lifecycle of the Influenza A virus contributing to the acidification necessary for viral RNA release.
Biological function summary
S31 is integral to the influenza virus replication process. It does not work alone but interacts with other viral proteins to form a functional ion channel complex. This complex is critical in modulating the internal pH of viral particles which subsequently assists in viral genome release into the host cell cytoplasm. The activity of S31 is pivotal for the successful entry of the virus into the host cell and its replication cycle.
Pathways
The functional role of S31 connects to the vesicular acidification and M2-mediated ion transport pathways. It is closely associated with the M1 protein of the Influenza A virus contributing to the virus's ability to hijack cellular machinery. These pathways facilitate the release of viral ribonucleoproteins into the cytoplasm by altering the endosomal pH important for the continuation of the viral replication process.
S31 is directly associated with Influenza A infections causing seasonal flu outbreaks. Mutations in the S31 protein can lead to resistance to antiviral drugs such as amantadine complicating treatment strategies. It connects to the M1 protein through the viral lifecycle impacting the efficiency of viral assembly and dissemination. Understanding the mechanism of S31 opens avenues for developing targeted therapeutics that inhibit viral propagation.