Abcam, ab249133, Anti-Palladin antibody [EPR9460(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Palladin antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR9460(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249133 is the carrier-free version of
ab154827
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Palladin is a cytoskeletal protein that plays an important role in maintaining cell structure and function. It is often referred to simply by its name and it has a molecular weight of approximately 90 to 140 kDa depending on its isoform. Palladin is expressed in a variety of tissues but shows a higher presence in cells such as fibroblasts smooth muscle cells and cardiac tissues. This protein interacts with numerous actin-associated proteins and contributes to the organization of the actin cytoskeleton by promoting actin polymerization.
Biological function summary
Palladin interacts with at least four actin-binding proteins to influence cellular processes such as motility and adhesion. Palladin often forms part of larger protein complexes that regulate the cytoskeleton. Through these interactions it contributes to the maintenance of cell shape and the mechanical stability of cells. Palladin’s role extends to influencing cell signaling pathways that regulate cell movement and structural integrity.
Pathways
Researchers connect palladin functions with cytoskeletal organization and cell signaling. It participates in pathways that involve the RhoA signaling pathway and the mechanotransduction pathway. Palladin interacts closely with proteins such as profilin and VASP synergistically influencing actin polymerization. These interactions are important for various cellular activities such as migration and morphogenesis.
Researchers have found palladin to be linked with conditions such as cancer and certain fibrosis. Altered expression levels of palladin can lead to enhanced metastatic potential in cancer cells. Additionally it exhibits associations with BRCA1 in breast cancer where mutations can alter cytoskeletal dynamics thereby affecting cell proliferation and migration. Palladin's overexpression is also observed in pancreatic cancer connecting it to actin-associated proteins that influence disease progression.