Abcam, ab249321, Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Ndufs1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11522(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, ICC/IF, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249321 is the carrier-free version of
ab157221
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ndufs1 known as NADH:ubiquinone oxidoreductase core subunit S1 or complex I 75 kDa subunit forms a significant part of the mitochondrial electron transport chain. This protein shows a mass of 75 kDa and is primarily found in mitochondria among various tissues. Ndufs1 plays an important role in transferring electrons from NADH to ubiquinone facilitating proton translocation across the inner mitochondrial membrane. With its function it contributes to the creation of a proton gradient essential for ATP synthesis.
Biological function summary
Ndufs1 facilitates the primary catalytic activity of the NADH dehydrogenase complex also found within the mitochondrial complex I. By being part of this large multi-subunit complex it helps in electron flow which essential for cellular respiration. Proper functioning of Ndufs1 becomes necessary for maintaining adequate energy production through ATP synthesis in cells impacting energy-dependent processes significantly.
Pathways
Ndufs1 acts mainly within the oxidative phosphorylation pathway. This pathway is indispensable for energy conversion in cells prominently featuring NADH dehydrogenase (ubiquinone). It relates closely with other critical proteins like Ndufa9 and Ndufb8 as these proteins also integrate into the mitochondrial complex I ensuring effective electron transport and energy yield in cells. The pathway links metabolic flux to energy production keeping cellular operations efficient.
Ndufs1 mutations correlate with mitochondrial diseases such as Leigh syndrome and mitochondrial complex I deficiency. These conditions often manifest neurological and muscular symptoms due to impaired mitochondrial function. Ndufs1 connections with proteins like Ndufv1 and additional complex I subunits emphasize its role in regular mitochondrial activity making its study relevant for understanding the pathological basis of these disorders. Researchers focus on Ndufs1 to unravel its impact on cellular metabolism and potential therapeutic targets.