Abcam, ab249346, Anti-NOTCH4 antibody [EPNCIR101B] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal NOTCH4 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPNCIR101B,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Data from our lab shows that this antibody does not work in WB on Human samples but does react with endogenous mouse protein. Other species have not been tested.

Product details:
ab249346 is the carrier-free version of
ab166605
This antibody was developed as part of a collaboration between the National Cancer Institutes Center for Cancer Research and the lab of Robert Callahan.
View antibodies from NCI Center for Cancer Research Collaboration
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please
contact us
for more information.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NOTCH4 also known as Neurogenic locus notch homolog protein 4 is a member of the NOTCH family of receptors. It has a molecular mass of approximately 301 kDa. This protein is expressed in various tissues such as the heart lungs and brain reflecting its involvement in numerous physiological processes. NOTCH4 is a type I transmembrane protein that participates in signal transduction by interacting with adjacent cells through its extracellular domain. It undergoes proteolytic cleavages that release its intracellular domain which then translocates to the nucleus to regulate gene transcription.
Biological function summary
NOTCH4 plays a fundamental role in cell differentiation proliferation and apoptosis. It is part of the NOTCH signaling pathway which involves other receptors like NOTCH1 NOTCH2 and NOTCH3. These receptor-ligand interactions help control the lineage and development of cells during embryogenesis and maintain homeostasis in adult tissues. The NOTCH pathway is important for vascular development and maintaining the vascular system's integrity.
Pathways
NOTCH4 is integral to the NOTCH signaling pathway and the angiogenesis pathway. In the NOTCH signaling activated NOTCH4 interacts with proteins like RBPJ to initiate specific transcriptional programs. It also plays a critical role in the angiogenesis pathway by modulating interactions necessary for endothelial cell behavior and blood vessel formation. These pathways allow NOTCH4 to coordinate with other family members like NOTCH2 to ensure proper cellular outcomes.
Aberrant NOTCH4 signaling connects to lung adenocarcinoma and ischemic stroke. In lung adenocarcinoma NOTCH4 dysregulation may interact with pathways involving proteins like KRAS leading to tumorigenesis. In ischemic stroke altered NOTCH4 function impacts angiogenesis potentially disturbing the blood flow and contributing to stroke pathology alongside other signaling molecules such as VEGF. Proper function and regulation of NOTCH4 appear critical for preventing these disease states.