Abcam, ab249398, Anti-gamma Adaptin antibody [EPR9875(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal gamma Adaptin antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR9875(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249398 is the carrier-free version of
ab167153
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Gamma adaptin also known as AP1G1 or AP-1 gamma subunit is a protein component of the adaptor protein complex 1 (AP-1). It has a molecular mass of approximately 91 kDa and functions in the Golgi apparatus as well as in the endosomes. This protein is important for intracellular transport mechanisms and influences the sorting and trafficking of various cargo proteins. Gamma adaptin is expressed in a range of tissues in the human body where it directs specific pathways of protein trafficking and plays a major role in maintaining cellular function.
Biological function summary
Gamma adaptin facilitates the recruitment of clathrin and other cargo proteins forming an important part of the AP-1 complex. AP-1 mediates the sorting of lysosomal enzymes and other proteins from the trans-Golgi network to endosomes and lysosomes. Through interactions with cytosolic adaptor proteins gamma adaptin contributes to vesicle formation and cargo selection. By participating in the correct shipping and transport of proteins within the cell it maintains cellular homeostasis and regulates vesicular trafficking.
Pathways
Gamma adaptin plays significant roles in both clathrin-mediated endocytosis and vesicular trafficking pathways. Within these pathways gamma adaptin interacts with proteins like clathrin and other adaptor proteins such as beta adaptin and mu adaptin. These interactions ensure the proper transport of proteins between cellular organelles. Gamma adaptin ensures precise intracellular protein distribution and impacts cellular communication processes. The interplay between gamma adaptin and other transport proteins is fundamental for maintaining efficient cellular operations.
Gamma adaptin has been linked to certain conditions such as atherosclerosis and neurological disorders. It interacts with proteins like Hermansky-Pudlak syndrome 1 (HPS1) which plays a role in the development of these diseases. Abnormalities in the function or expression of gamma adaptin can lead to improper protein trafficking contributing to disease progression. Understanding the relationships between gamma adaptin and these diseases offers potential insights for novel therapeutic strategies targeting the faulty shipping and transport mechanisms within the cell.