Abcam, ab249438, Anti-PNMT antibody [EPR7547(2)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal PNMT antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR7547(2),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249438 is the carrier-free version of
ab167427
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please
contact us
for more information.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein PNMT also known as Phenylethanolamine N-methyltransferase functions as an enzyme responsible for the methylation of norepinephrine to synthesize epinephrine. It belongs to the methyltransferase family and weighs approximately 30 kDa. PNMT is highly expressed in the adrenal medulla particularly in chromaffin cells but also found in smaller amounts in some parts of the brain such as the medulla oblongata and retina. This distribution supports its role in catecholamine biosynthesis and regulation.
Biological function summary
The conversion of norepinephrine to epinephrine increases the functionality of PNMT in stress response mechanisms. PNMT plays an important role in the synthesis of catecholamines which are essential for modulating the fight-or-flight response. It does not form part of a larger protein complex but interacts with substrates and cofactors directly to catalyze its reactions. PNMT's activity is carefully regulated by factors including substrate availability and enzyme expression levels in tissues where it operates.
Pathways
Norepinephrine to epinephrine synthesis conducted by PNMT marks the final step in the catecholamine biosynthetic pathway. This pathway is critical in managing cardiovascular and metabolic functions. The protein connects extensively with enzymes such as tyrosine hydroxylase and dopamine β-hydroxylase within this pathway which are involved in prior steps of catecholamine synthesis. PNMT's activity and regulation are therefore essential for maintaining homeostasis in stress and energy metabolism.
PNMT connects directly to conditions like hypertension and certain phaeochromocytomas which arise from catecholamine excess or imbalance. Elevated levels of PNMT can be found in tumors linked to the adrenal medulla affecting the production of epinephrine. PNMT's interaction with enzymes like dopamine β-hydroxylase is relevant in understanding these disorders since imbalances in enzyme activity can lead to disrupted catecholamine synthesis contributing to disease pathogenesis.