Abcam, ab249465, Anti-PPP2CB antibody [EPR11786(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal PP2AB antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11786(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249465 is the carrier-free version of
ab168371
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PPP2CB also known as protein phosphatase 2 catalytic subunit beta is an enzyme that plays an important role in cellular functions. It plays a catalytic role in dephosphorylating serine and threonine residues in various proteins. The alternative names used for PPP2CB include PP2Acβ and PP2A(C subunit)β. This protein has a mass of approximately 35 kDa and is expressed in a variety of tissues with significant presence in heart skeletal muscle and brain. Researchers note its expression in both cytoplasmic and nuclear locations within cells.
Biological function summary
The protein phosphatase activity of PPP2CB influences many cellular processes. It is a vital part of the protein phosphatase 2 (PP2A) complex which is a major serine/threonine phosphatase in cells. Through its involvement in the PP2A complex PPP2CB regulates cellular processes like cell growth division and apoptosis. The PP2A complex of which PPP2CB is a component remains essential to signaling pathways contributing to the dynamic behavior of cellular phosphorylation-dephosphorylation cycles.
Pathways
Protein phosphatase 2's function aligns PP2CB with key signaling routes such as the MAPK and Wnt pathways. In the MAPK pathway PP2A complex dephosphorylates specific kinases which controls cellular responses to external stimuli. Within the Wnt pathway the regulatory actions of PP2A maintain the appropriate level of pathway activity. The roles of PPP2CB in these pathways connect it to other related proteins like B56 family regulatory subunits in signal transduction networks.
Disruptions in PPP2CB activity link to several conditions including cancer and neurodegenerative diseases. The dysregulation of PPP2CB in cancer is often associated with altered cell proliferation and survival as PP2A is a tumor suppressor complex. In neurodegenerative diseases particularly Alzheimer's aberrant PPP2CB activity disrupts phosphorylation states of tau protein contributing to disease pathogenesis. PPP2CB interacts with other significant proteins like tau in Alzheimer's highlighting its importance in maintaining cellular and neurological health.