Abcam, ab249587, Anti-EIF5 antibody [EPR12140(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal EIF5 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR12140(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:Flow Cyt (Intra), ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249587 is the carrier-free version of
ab170915
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The eukaryotic translation initiation factor 5 (EIF5) plays an important role in the initiation phase of protein synthesis. EIF5 also known as eIF-5 is a protein that exhibits a molecular weight of approximately 49 kDa. It functions as a GTPase-activating protein that triggers the hydrolysis of GTP bound to the eukaryotic initiation factor 2 (eIF2) an essential step for the formation of the 80S ribosome complex during translation. EIF5 expression occurs widely across various tissues indicating its necessity for basic cellular functions.
Biological function summary
EIF5 facilitates the translation initiation process which is critical for protein synthesis. It is an integral component of the multifactor eIF2 complex that ensures the accuracy of start codon recognition in mRNA. This complex assembly involves the association with other initiation factors like eIF1 and eIF3 orchestrating mRNA scanning and the selection of the start site for protein synthesis. EIF5's role in regulating protein biosynthesis links it to the maintenance of cellular homeostasis.
Pathways
EIF5 serves a critical part in the translation initiation pathway. This pathway is essential for general protein biosynthesis directly impacting cellular growth and proliferation. It closely collaborates with related factors like eIF2 and eIF2B within this pathway to modulate translation in response to cellular stress and nutrient availability. Additionally EIF5 intersects the mTOR signaling pathway where it interacts with proteins that coordinate cell growth and metabolism under various physiological conditions.
EIF5 alterations can lead to disruptions in protein synthesis connecting it to certain human diseases like cancer. Some studies indicate a link between EIF5 dysregulation and tumorigenesis as faults in translation regulation can drive abnormal cell proliferation. Beyond cancer EIF5 may also intersect with neurodegenerative diseases given the translation regulation's importance in neuronal function. It can associate with other factors such as eIF2α wherein heightened EIF5 activity influences stress responses pivotal in disease contexts.