Abcam, ab249602, Anti-TUBB2A+TUBB2B antibody [EPR11843] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal TBB2A antibody. Carrier free. Suitable for IHC-P, IP, Flow Cyt (Intra), WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11843,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IP, Flow Cyt (Intra), IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249602 is the carrier-free version of
ab170931
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TUBB2A and TUBB2B are members of the tubulin family which forms microtubules. Together they play an important role in providing structural support within cells. TUBB2A is sometimes referred to as tubulin beta-2A chain whereas TUBB2B is known as tubulin beta-2B chain. Both TUBB2A and TUBB2B have a molecular mass of approximately 50 kDa. These proteins are expressed mainly in neurons contributing to the formation of the microtubule network essential for cell shape intracellular transport and cell division.
Biological function summary
TUBB2A and TUBB2B are integral to the assembly of microtubules which are part of the cytoskeleton complex. These microtubules provide a framework that maintains cell shape and facilitates the transport of organelles and vesicles within neurons. In the neuronal environment TUBB2A and TUBB2B are critical for axon guidance and neuronal migration aiding the proper wiring of the nervous system during development. These proteins are also involved in stabilizing the dynamic instability of microtubules ensuring that they can undergo rapid growth and shrinkage as needed.
Pathways
TUBB2A and TUBB2B play a role in the cytoskeletal regulation pathway which controls cellular architecture and movement. They work in conjunction with other tubulin isotypes to polymerize microtubules that are involved in the mitotic spindle formation during cell division. Additionally these proteins interact with microtubule-associated proteins (MAPs) for the regulation of microtubule stability and dynamics. TUBB2A and TUBB2B also relate to the neuronal development pathway aligning with proteins like MAP2 and tau that stabilize microtubule structures specifically in neurons.
Mutations in TUBB2A and TUBB2B have been linked to developmental brain disorders such as polymicrogyria and pachygyria. These conditions are characterized by abnormal brain development which can lead to intellectual disability epilepsy or other neurological problems. TUBB2A and TUBB2B mutations may disrupt the function of related proteins like GABRB2 which is involved in neurotransmission contributing to altered neuronal connectivity and function. Their dysfunction affects microtubule dynamics resulting in improper neuronal migration and brain formation.