Abcam, ab249614, Anti-Myelin expression factor 2 antibody [EPR10774(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Myelin expression factor 2 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR10774(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:Flow Cyt (Intra), WB, ICC/IF, IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249614 is the carrier-free version of
ab170946
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Myelin expression factor 2 also known as MYEF2 is a transcription repressor that regulates the transcription of myelin-specific genes. It plays an important role in the control of myelin production and is primarily expressed in the nervous system especially in oligodendrocytes. The factor weighs approximately 100 kDa and its expression is critical at the periods of neuronal development. MYEF2 demonstrates its activity within the cell nucleus where it functions directly on DNA to modulate gene expression.
Biological function summary
MYEF2 influences the differentiation and maturation of oligodendrocytes. These cells are responsible for forming the myelin sheath in the central nervous system which is essential for rapid impulse conduction along nerve fibers. MYEF2 does not act alone but integrates into complexes with other proteins such as histone deacetylases to exert its repressive function. This interaction fine-tunes oligodendrocyte activity ensuring proper neuronal insulation and function.
Pathways
MYEF2 interacts with the signaling cascades of myelination and neural development. In myelination pathways MYEF2 collaborates closely with proteins like SOX10 and OLIG1 both of which are vital for oligodendrocyte differentiation and maturation. Additionally MYEF2 associates indirectly with the Notch pathway affecting neural progenitor cell fate decisions. The integration of these pathways guarantees the precise regulation of myelin gene expression and hence normal nervous system function.
MYEF2's dysregulation links to demyelinating diseases such as multiple sclerosis and certain leukodystrophies. In multiple sclerosis improper MYEF2 activity can lead to defective myelin production exacerbating disease symptoms and progression. MYEF2's function could also relate to disturbances in proteins like myelin basic protein (MBP) which is important for myelin integrity. Understanding MYEF2’s role in these disorders may offer insights for therapeutic targets to manage and treat neurological conditions involving myelin damage.