Abcam, ab249634, Anti-NAF1 antibody [EPR11516] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal NAF1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11516,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249634 is the carrier-free version of
ab171078
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NAF1 also known as NHP2L1 is a protein involved in essential mechanical processes in the cell. It weighs approximately 83 kDa and mainly localizes to the nucleus specifically within Cajal bodies and nucleolus. The protein plays a role in the assembly and maturation of small nucleolar ribonucleoproteins (snoRNPs) which are important for RNA processing. NAF1 expression occurs across diverse tissues indicating its importance in general cellular function.
Biological function summary
NAF1 is a component of the H/ACA ribonucleoprotein complex. It participates in guiding the chemical modification of ribosomal and other small nuclear RNAs. NAF1 acts as a scaffold facilitating the attachment of small RNAs and interacting with other proteins like NOP10 and GAR1 within the snoRNP complex. This organization is essential for the stability and function of the complex which impacts RNA folding and maturation ultimately regulating protein synthesis.
Pathways
The involvement of NAF1 in RNA modification is critical in the RNA splicing and ribosome biogenesis pathways. It plays a role in the maturation of ribosomal RNA an important step in assembling functioning ribosomes. NAF1's activity indirectly influences the translation process with links to additional proteins such as dyskerin which also have essential functions in ribosome assembly and RNA stability.
Studies have connected NAF1 function to conditions like dyskeratosis congenita and certain cancers. The link to dyskeratosis congenita arises from its association with the stability and function of telomerase RNA a pathway shared with proteins such as dyskerin. In some cancers abnormalities in NAF1 expression or function have been implicated in tumor progression highlighting its potential role in maintaining genomic stability and cellular proliferation.