Abcam, ab249663, Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal QPRT antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11941(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:Flow Cyt (Intra), IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249663 is the carrier-free version of
ab171944
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
QPRT also known as Nicotinate-nucleotide pyrophosphorylase [carboxylating] or Quinolinate phosphoribosyltransferase functions as an important enzyme in the kynurenine pathway a metabolic pathway leading to the production of nicotinamide adenine dinucleotide (NAD+). The enzyme catalyzes the conversion of quinolinate to nicotinic acid mononucleotide. QPRT has a molecular mass of approximately 34 kDa. It is expressed in various tissues most notably in the liver kidney and brain.
Biological function summary
QPRT influences the synthesis of NAD+ which is essential for cellular redox reactions and energy metabolism. The enzyme operates as a monomer and does not typically form part of a larger complex. However its activity is important in maintaining NAD+ levels which can impact a wide range of cellular processes such as DNA repair signal transduction and metabolism.
Pathways
QPRT holds a significant role in the kynurenine pathway and NAD+ biosynthesis. This pathway includes several enzymes such as kynurenine 3-monooxygenase and kynureninase which collaboratively contribute to the degradation of tryptophan ultimately affecting the levels of important coenzymes and neurotransmitters. The NAD+ biosynthesis pathway involving QPRT also interacts with pathways for ATP generation connecting with other metabolic processes.
QPRT's malfunction links to neurological disorders such as Alzheimer's disease due to its role in NAD+ metabolism and potential effects on neuronal health and energy supply. Additionally alterations in QPRT activity have associations with liver diseases considering its expression and function in metabolic pathways within hepatic tissues. Interaction with proteins like kynurenine aminotransferase highlights its involvement in maintaining metabolic balance within these conditions.