Abcam, ab249714, Anti-TAF5L antibody [EPR11445] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal TAF5L antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11445,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249714 is the carrier-free version of
ab172621
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TAF5L also known as Transcription initiation factor TFIID subunit 5-like is a protein that plays an important role in gene expression through its involvement in the transcription machinery. It weighs approximately 80 kDa and is composed of 787 amino acids. TAF5L is expressed in various tissues with higher levels in the spleen thymus and bone marrow. This expression pattern indicates its role in processes that require precise transcription regulation.
Biological function summary
TAF5L functions as a fundamental unit of the transcription factor IID (TFIID) complex. This complex comprises several other TAF proteins and the TATA-binding protein (TBP) orchestrating the assembly of the preinitiation complex (PIC) on promoters. By stabilizing interactions between these proteins TAF5L facilitates the precise transcription initiation of various genes. Its role in the TFIID complex makes it essential for the recruitment of RNA polymerase II to specific DNA regions.
Pathways
TAF5L actively participates in the RNA polymerase II transcription initiation pathway and the regulation of cellular response to DNA damage. Within these pathways its interaction with other TAF subunits and the TBP coordinates transcription activities. TAF5L specifically teams up with TAF6 to support the structural integrity of the TFIID complex ensuring effective transcription of genes in response to developmental and environmental signals.
TAF5L shows connections to certain cancers including colorectal and breast cancer. Alterations in TAF5L expression or function can lead to dysregulation of gene transcription contributing to tumorigenesis and cancer progression. The protein's association with the p53 signaling pathway responsible for maintaining genomic stability further highlights its importance in cancer. Additionally disruptions in TAF5L interactions with other TAF proteins or TBP may impact cell cycle control further linking it to cancer development.