Abcam, ab249822, Anti-MMAB antibody [EPR8695] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal MMAB antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR8695,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249822 is the carrier-free version of
ab174831
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MMAB also known as methylmalonic aciduria type B protein plays a critical role in the conversion of certain molecules within the mitochondria. This protein weighing approximately 38 kDa assists in the conversion of vitamin B12 into adenosylcobalamin an active coenzyme form. MMAB is present in many tissues with significant expression in liver and kidney where significant mitochondrial activities occur. Methylmalonyl-CoA a byproduct needing processing for energy production relies on MMAB for transformation.
Biological function summary
MMAB is indispensable for vitamin B12 metabolism and mitochondrial function. This protein acts within a larger enzymatic complex that converts methylmalonyl-CoA to succinyl-CoA. By playing a role in this conversion MMAB facilitates the Krebs cycle an essential biochemical pathway for energy production. Its interaction with other proteins like methylmalonyl-CoA mutase ensures proper biochemical transformations highlighting its importance in metabolic processes.
Pathways
Methylmalonic acidemia results from disruptions in the MMAB-mediated pathway notably affecting energy metabolism. This protein integrates into the mitochondrial pathway that handles odd-chain fatty acids and amino acid breakdown. The effective collaboration of MMAB with methylmalonyl-CoA mutase ensures efficient functioning within these pathways linking MMAB to essential metabolic activities like gluconeogenesis and energy production.
Mutations in MMAB contribute to methylmalonic acidemia a metabolic disorder characterized by accumulation of methylmalonic acid in the body. This condition can lead to developmental delay metabolic crisis and other severe symptoms if untreated. Alongside methylmalonyl-CoA mutase MMAB mutations impact vitamin B12-related pathways highlighting their joint role in this genetic disorder. Early diagnosis and treatment targeting this pathway help mitigate symptoms related to these enzymatic dysfunctions.