Abcam, ab249824, Anti-GRASP65 antibody [EPR12439] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal GRASP65 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR12439,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:ICC/IF, Flow Cyt (Intra), IHC-P, IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249824 is the carrier-free version of
ab174834
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GRASP65 also known as Golgi reassembly stacking protein 65 is an important Golgi apparatus-associated protein. It weighs approximately 74 kDa and is expressed predominantly in mammalian cells. GRASP65 localizes to the cis-Golgi cisternae performing functions necessary for preserving the Golgi structure and facilitating protein trafficking. This protein plays a role in maintaining the Golgi ribbon during the cell cycle which is essential for proper cellular operation.
Biological function summary
GRASP65 participates in key processes related to Golgi stack organization and structure. It forms a complex with other Golgi proteins acting as an important scaffold protein during membrane tethering and linking adjacent Golgi membranes. GRASP65 influences Golgi dynamics by regulating Golgi stacking and membrane fusion events which are essential processes for the normal functioning of the secretory pathway.
Pathways
GRASP65 integrates into the mitotic Golgi fragmentation pathway and also influences the ER-to-Golgi transport pathway. It interacts with proteins such as GM130 and forms a bridge between vesicles and Golgi membranes influencing vesicle tethering and cisternal formation. In these roles GRASP65 helps coordinate Golgi disassembly and reassembly processes that are important during cell division to ensure proper Golgi inheritance among daughter cells.
GRASP65 displays relationships with certain neurodegenerative and cancerous conditions. Dysfunctional GRASP65 activity associates with Alzheimer's disease-related pathologies potentially linked through proteins such as tau which aggregates during disease progression. It also connects to certain cancers by misregulating Golgi structure suggesting a role in improper cell cycle control. Understanding GRASP65's function and regulation could offer insights into therapeutic strategies for these diseases.