Abcam, ab249859, Anti-Muted antibody [EPR11081(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Muted antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11081(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249859 is the carrier-free version of
ab175205
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Muted protein also known as MUTED plays a role in the trafficking and sorting of proteins in vesicles. This small protein with an approximate molecular mass of 22 kDa mainly expresses in melanocytes but researchers have also observed it in other cell types. It functions closely with other proteins involved in the regulation of melanosome biology. The expression pattern of Muted suggests it has specific roles in pigmentation and possibly related cellular processes.
Biological function summary
Muted influences important cellular functions by forming part of the biogenesis of lysosome-related organelles complex-1 (BLOC-1). This complex which includes eight subunits is vital for the generation of lysosome-related organelles such as melanosomes and platelet-dense granules. Muted's role in the complex affects the specialized organelles essential for pigmentation and other related cellular processes. Without proper functioning of BLOC-1 various cellular activities reliant on functional organelles face impairment.
Pathways
Muted and other molecules participate in the endosomal-lysosomal pathway and the melanosome biogenesis pathway. These pathways are important for the formation of lysosome-related organelles. Alongside proteins like Pallidin and Cappuccino Muted influences the movement and formation of vesicles required for transporting cargos to their destination within cells. Proper coordination within these pathways ensures cellular components reach the right location influencing pigmentation and other cell-specific functions.
Muted's malfunction associates with conditions such as Hermansky-Pudlak syndrome and oculocutaneous albinism type 2. These disorders result from defects in the biogenesis of lysosome-related organelles. Muted and associated proteins like Melanophilin and Myosin Va guide the transport mechanisms impaired in these disease states leading to characteristic symptoms like hypopigmentation and bleeding disorders. Understanding the Muted protein helps in deciphering the molecular basis of these conditions and aids in the development of potential interventions.