Abcam, ab249889, Anti-SAP18 antibody [EPR12956(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal SAP18 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR12956(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Rat, Human,
Applications:WB, IP, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249889 is the carrier-free version of
ab175920
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The target SAP18 also known as Sin3A-associated protein 18 kDa functions as a part of a critical molecular machinery. With an approximate mass of 18 kilodaltons SAP18 acts as a component of the Sin3A corepressor complex which has roles in the regulation of gene expression. It is expressed in a variety of tissues with significant expression detected in neuronal cells. Through its interaction with other proteins in the complex SAP18 contributes to the repression of specific genes by altering chromatin structure.
Biological function summary
Proteins such as SAP18 influence essential processes by serving as a component of the Sin3A/histone deacetylase (HDAC) complexes. These complexes facilitate transcriptional repression by removing acetyl groups from histone proteins which leads to chromatin condensation and reduced gene activity. SAP18 as part of these complexes aids in regulating genes involved in cell differentiation apoptosis and development. Its role in these processes highlights its importance in maintaining cellular functions and homeostasis.
Pathways
The role of SAP18 in gene expression regulation supports its involvement in key pathways such as cell cycle control and signal transduction. In the context of the cell cycle pathway SAP18 and its complexes work closely with other regulatory proteins like Rb and E2F to ensure proper cell cycle progression by modulating transcription of cycle-dependent genes. In signal transduction pathways SAP18 has the capacity to interact with proteins like HDAC1 influencing the downstream signaling that impacts cell fate decisions.
Researchers have linked SAP18 aberrations to conditions such as cancer and neurodegenerative diseases. In cancer dysregulation of SAP18 expression or function can lead to uncontrolled cell proliferation due to a failure in proper gene repression involving interactions with proteins like HDAC1 and Rb. In neurodegenerative diseases altered SAP18 activity can contribute to neuronal cell death with connections to proteins within the Sin3A/HDAC complex that are important in neuronal survival pathways. These associations highlight SAP18 as a potential therapeutic target in relevant pathological conditions.