Abcam, ab249898, Anti-MRPS35 antibody [EPR11730] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal MRPS35 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11730,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IP, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249898 is the carrier-free version of
ab175931
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MRPS35 also known as 28S ribosomal protein S35 or mitochondrial ribosomal protein S35 is a component of the small subunit of the mitochondrial ribosome. This protein is integral to mitochondrial protein synthesis and weighs approximately 39 kDa. It is expressed widely in human tissues but shows higher expression in tissues with high energy demand like skeletal muscle and heart. MRPS35 plays a mechanical role in facilitating the translation of mitochondrial mRNA to proteins necessary for mitochondrial function.
Biological function summary
MRPS35 is essential in mitochondrial ribosome assembly and protein synthesis. As part of the mitochondrial ribosomal small subunit it contributes to the assembly of ribosomes and the translation of essential proteins for mitochondrial energy production. This protein partners with other mitochondrial ribosomal proteins and plays a role in the synthesis of oxidative phosphorylation components an important metabolic pathway for ATP production.
Pathways
MRPS35 is significant in cellular respiratory processes. It plays a role in the oxidative phosphorylation pathway which is important for ATP production. MRPS35 interacts with other mitochondrial ribosomal proteins like MRPS30 to ensure efficient assembly and function of the mitochondrial ribosome. The proper synthesis of mitochondrial proteins is critical for maintaining the electron transport chain's function.
MRPS35 relates to certain mitochondrial disorders and possibly metabolic syndromes. Defects in mitochondrial protein synthesis can lead to conditions such as mitochondrial myopathy and MELAS syndrome (Mitochondrial Encephalopathy Lactic Acidosis and Stroke-like episodes). MRPS35 through its role in mitochondrial protein synthesis also has connections with proteins like MRPL44 which is another component of the mitochondrial ribosome where defects in these proteins can potentially exacerbate mitochondrial dysfunction.