Abcam, ab249931, Anti-ISL-2 antibody [EPR12785] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ISL-2 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR12785,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249931 is the carrier-free version of
ab176576
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ISL-2 also known as ISLET2 or Islet-2 is a transcription factor with an approximate mass of 39 kDa. It belongs to the LIM/homeobox family and primarily functions by binding DNA to regulate gene transcription. This protein is essential in the central nervous system and is also expressed in the developing spinal cord. In these tissues ISL-2 facilitates the differentiation and maturation of specific neuronal populations by influencing the transcription of downstream target genes.
Biological function summary
As part of a complex network of transcription factors ISL-2 plays an important role in neuronal development. It controls genes involved in motor neuron differentiation and axonal guidance. ISL-2 influences the identity and function of neurons participating in the formation of neural circuits. The protein's regulatory capacity ensures correct neuronal patterning impacting the functioning of developing and mature nervous systems.
Pathways
ISL-2 interacts with signaling pathways related to neuronal specification such as the Hedgehog and Wnt pathways. These pathways are important for the regulation of neural tube development and organization of neuronal structures. In the Wnt pathway ISL-2 interacts with proteins like β-catenin to modulate gene expression important for neuronal identity. Its involvement in these pathways reinforces the protein's function in maintaining nervous system integrity.
ISL-2 has implications in neurodegenerative diseases and spinal muscular atrophy. Mutations or dysregulation of ISL-2 expression can affect motor neuron health leading to progressive loss of motor function. It shows connections with other proteins involved in neurodegeneration such as SMN (survival motor neuron protein) which plays a role in the disease's pathology. Understanding ISL-2's regulation and interactions can offer insights into therapeutic strategies for such disorders.