Abcam, ab250033, Anti-Mkln1 antibody [EPR13519] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal MKLN1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR13519,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250033 is the carrier-free version of
ab178403
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Mkl1 also known as megakaryoblastic leukemia (translocation) 1 is a protein encoded by the MKL1 gene. The mass of Mkl1 approximately measures around 130 kDa. This protein is expressed in multiple tissues but it exhibits higher expression in the heart and skeletal muscle. Mkl1 localizes mainly in the nucleus where it functions as a coactivator of serum response factor (SRF) playing an important role in actin dynamics and transcriptional regulation.
Biological function summary
Mkl1 coordinates transcriptional programs required for cell differentiation and cytoskeletal organization. As a component of the MRTF-SRF complex Mkl1 regulates genes involved in actin cytoskeleton remodeling. This function impacts cell migration adhesion and morphology influenced by external signals. Additionally it plays a role in the regulation of smooth muscle and cardiac-specific gene expression which highlights its importance in muscle physiology.
Pathways
Mkl1 serves an important function in the RhoA-actin signaling pathway. This pathway controls the organization of the actin cytoskeleton and is connected with several cellular functions including cell shape and motility. Mkl1 interacts closely with SRF as well as other proteins like RhoA GTPase which are vital for transmitting signals that promote actin polymerization and cell movement. This pathway integration contributes to understanding how extracellular cues translate into cytoskeletal rearrangements.
Mkl1 has significant connections to megakaryoblastic leukemia and cardiac hypertrophy. In leukemia a chromosomal translocation involving Mkl1 and another protein MOZ leads to the production of an oncogenic fusion protein that causes dysregulation of normal gene expression contributing to malignancy. Additionally upregulated Mkl1 expression links to cardiac hypertrophy where it facilitates pathological gene expression in heart muscle cells. The role of Mkl1 in these disorders highlights its significance in disease progression and susceptibility.