Abcam, ab250142, Anti-RNF21/IFP1 antibody [EPR11305(2)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal RNF21/IFP1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11305(2),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250142 is the carrier-free version of
ab180130
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RNF21 also known as IFP1 is a human protein with a molecular weight of approximately 43 kDa. It belongs to the RING finger protein family functioning as an E3 ubiquitin ligase. Members of this family typically regulate protein degradation through the ubiquitin-proteasome system. RNF21 is widely expressed in various tissues including the brain liver and heart. This expression suggests important roles across different physiological contexts.
Biological function summary
The E3 ubiquitin ligase activity of RNF21 contributes to the regulation of protein homeostasis. RNF21 potentially participates in forming larger protein complexes which could be involved in cellular processes like cell cycle regulation. By tagging specific proteins for degradation RNF21 maintains cellular protein equilibrium. This process impacts various cellular functions including signal transduction and cellular stress responses.
Pathways
The function of RNF21 integrates within the ubiquitin-proteasome pathway and connects to protein turnover. This pathway involves numerous proteins such as ubiquitin-conjugating enzymes which work alongside RNF21. Additionally RNF21 may intersect with signaling pathways related to protein stability where it collaborates with proteins like SCF complexes which also function in protein degradation.
RNF21 has connections to neurodegenerative diseases such as Alzheimer's disease where protein aggregation becomes problematic. Aberrant protein degradation can exacerbate these disease conditions. RNF21's interactions with proteins like tau which is involved in Alzheimer's pathology illustrate its potential influence. Moreover its role in inflammatory responses might link RNF21 to disorders like rheumatoid arthritis where improper protein regulation plays a role.