Abcam, ab250224, Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Spry-2 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR4318(2)(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Rat,
Applications:WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250224 is the carrier-free version of
ab180527
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Spry-2 also known as Sprouty homolog 2 is an intracellular protein involved in the regulation of signaling pathways. It has a molecular weight of approximately 35 kDa. This protein expresses in tissues such as the lung kidney and colon. Mainly Spry-2 acts through interaction with other proteins to inhibit or modulate various cellular processes such as growth and differentiation.
Biological function summary
Sprouty homolog 2 functions by mediating negative feedback in signaling pathways. It plays a role in controlling cellular responses to growth factors. Although not part of a large protein complex it interacts with specific proteins to exert its biological effects. For instance Spry-2 interacts with components of the receptor tyrosine kinase signaling pathway. This interaction helps fine-tune cell signaling responses preventing excessive cell proliferation.
Pathways
Sprouty homolog 2 acts within the MAPK/ERK signaling pathway and the fibroblast growth factor (FGF) pathway. In these pathways Spry-2 functions as an inhibitor thereby maintaining proper cellular regulation. It interacts with proteins like Raf and inhibits their activities ensuring balanced signal transduction. In the MAPK/ERK pathway this regulation by Spry-2 helps control cellular responses to external stimuli.
Abnormalities in Sprouty homolog 2 function relate to cancer and congenital anomalies. Altered expression or mutations in Spry-2 have associations with colorectal cancer partly due to its role in cell signaling regulation. Spry-2 connects to proteins like Ras known for their involvement in oncogenic pathways. Similarly variations in Spry-2 activity can lead to developmental disorders highlighting its importance in normal cellular function.