Abcam, ab250244, Anti-EBP1 antibody [EPR14568(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal EBP1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14568(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250244 is the carrier-free version of
ab180603
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The EBP1 protein also known as ErbB3-binding protein 1 plays an important role in cellular mechanisms. With a mass of about 39 kDa EBP1 is widely expressed in various tissues including the heart liver and brain. The protein acts as an RNA-binding protein and it has demonstrated influence in the regulation of ribosomal RNA synthesis and processing. EBP1 also interacts with multiple other proteins which aid in its regulatory roles in the cells.
Biological function summary
EBP1 functions as a part of the cellular machinery involved in growth and differentiation. It associates with the eukaryotic translation initiation factor 4G (eIF4G) as part of a complex which modulates the translation of certain messenger RNAs. Furthermore EBP1 can function as a transcriptional co-repressor in the nucleus where it influences gene expression by interacting with DNA. Its activity in these roles highlights its important function in cell cycle regulation and cellular proliferation.
Pathways
Many signaling mechanisms engage EBP1. The PI3K/AKT pathway sees participation from EBP1 where it downstream affects cell survival and growth. EBP1 also interacts with the NF-κB pathway contributing to the regulation of immune and stress responses. In these pathways interactions with key proteins like AKT and NF-κB p65 emphasize its role in integrating signaling cues for responses to environmental stimuli.
EBP1 has shown associations with cancer and neurodegenerative diseases. Its connection with cancer is evident as EBP1 influences cell proliferation and can affect tumor suppressor pathways sometimes interacting with proteins like HER2. In neurodegenerative diseases deregulation of EBP1 function has been seen in conditions such as Alzheimer's potentially interacting with proteins involved in tau phosphorylation. Both examples demonstrate the biological significance of EBP1 in maintaining normal physiological function and its contributions to pathology when dysregulated.