Abcam, ab250250, Anti-Nogo antibody [EPR12265] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal RTN4 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR12265,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IP, IHC-P, WB, Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250250 is the carrier-free version of
ab180847
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Nogo protein also known as RTN4 or Nogo-A is a member of the reticulon family with an approximate mass of 200 kDa. It is known for its inhibitory role in axonal regeneration acting as a potent neurite outgrowth inhibitor. The Nogo protein is widely expressed in the central nervous system especially in oligodendrocytes and the endoplasmic reticulum of neurons. It is an important target in neurobiology due to its involvement in regulating axonal growth.
Biological function summary
Nogo protein plays a critical role in the central nervous system by maintaining the stability of neuronal circuits and preventing excessive or aberrant plasticity. It achieves this through its association with membrane-bound complexes. The Nogo-A variant in particular interacts with the Nogo receptor (NgR1) complex to mediate its effects on axon outgrowth inhibition. This interaction is important for maintaining neural structural integrity under normal physiological conditions.
Pathways
The Nogo protein is integrated into neurite outgrowth-inhibitory pathways influencing neural connectivity. It participates mainly in the RhoA-ROCK signaling pathway a pathway known for its role in modulating the cytoskeleton and neuronal morphology. Through this pathway Nogo interacts with several proteins including the p75 neurotrophin receptor (p75^NTR) mediating downstream effects on cell shape and motility. The regulation of this pathway is significant for understanding axonal regeneration and growth inhibition.
The Nogo protein is relevant in conditions such as spinal cord injury and multiple sclerosis. In spinal cord injuries upregulated Nogo-A expression contributes to the inhibition of axonal regeneration and functional recovery. Similarly in multiple sclerosis Nogo protein's interaction with the myelin-associated inhibitors may exacerbate damage or delay in repair linked with impaired oligodendrocyte function. This connection highlights the potential therapeutic importance of targeting Nogo interactions to promote neurological recovery and repair.