Abcam, ab250264, Anti-PDIA2/PDIP antibody [EPR12212] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal PDIA2/PDIP antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR12212,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250264 is the carrier-free version of
ab180931
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PDIA2 also known as PDIP is a protein disulfide isomerase with a prominent role in catalyzing protein folding through the isomerization of disulfide bonds. It is part of the broader family known as PDI proteins. PDIA2 has a mass of approximately 57 kDa and expression occurs mainly in the endoplasmic reticulum. Its enzymatic activity contributes to the maintenance of cellular homeostasis by ensuring proper protein conformation. Researchers have identified PDIA2 as a target for potential PDI inhibitors due to its critical function in protein folding.
Biological function summary
PDIA2 facilitates the correct formation of disulfide bonds which is essential for protein maturation and stability. This protein operates within a multi-component complex involved in oxidative protein folding regulating the redox state in the endoplasmic reticulum environment. By assisting in proper protein assembly PDIA2 maintains the equilibrium of protein synthesis folding and degradation suggesting its importance in cellular protein metabolism.
Pathways
Studies show that PDIA2 plays a role in the oxidative protein folding pathway which is part of the larger cellular response network to oxidative stress. The interaction of PDIA2 with other protein disulfide isomerases such as PDI and ERP57 highlights its role in this critical pathway. Furthermore PDIA2 is linked to the unfolded protein response pathway indicating its involvement in cell stress conditions where misfolded proteins accumulate.
PDIA2 has associations with metabolic disorders and neurodegenerative diseases. Its role in the proper folding of insulin and other secreted proteins links PDIA2 to diabetes-related conditions. Additionally research suggests connections between PDIA2 and neurodegenerative diseases like Alzheimer's where protein misfolding and aggregation play pivotal roles. PDIA2's relationship with proteins like amyloid precursor protein further corroborates its involvement in the pathological processes of such disorders.