Abcam, ab250269, Anti-RAB13 antibody [EPR14109(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal RAB13 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14109(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250269 is the carrier-free version of
ab180936
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RAB13 also known by the names YPT13 and RAB11FIP5 is a small GTPase from the RAB family with a molecular mass of approximately 23 kDa. It localizes to both the cytosol and various membrane compartments. Expressed broadly in different tissues including epithelial tissues and fibroblasts RAB13 plays an active role in vesicle trafficking processes. It primarily modulates the dynamics of vesicle formation transportation and fusion making it critical for maintaining cellular membrane identity and homeostasis.
Biological function summary
RAB13 influences cell surface dynamics by controlling the trafficking of proteins to the plasma membrane. It participates in forming complexes with other RAB proteins ensuring precise membrane delivery and recycling. As a result RAB13 assists in cell junction maintenance and tight junction stability enabling proper cell polarization and epithelial barrier function. This function is essential for processes like cell migration and tissue integrity.
Pathways
The role of RAB13 in intracellular trafficking links it to essential biological pathways such as the actin cytoskeleton regulation and the signaling of the epithelial-to-mesenchymal transition (EMT). In the actin regulation pathway RAB13 closely interacts with proteins like RHOA and CDC42 which assist in reorganizing the cytoskeleton essential for cell movement. For the EMT process RAB13 plays a part in modulating the cellular adherence and motility pathways which are critical during development and repair mechanisms.
The dysregulation of RAB13 expressions has connections to cancer progression and neurological disorders. Studies show that altered RAB13 activity can contribute to invasive cancer phenotypes such as in breast cancer by facilitating abnormal epithelial cell movement. Additionally improper RAB13 function links to neurological conditions where axonal transport is disrupted highlighting a connection with proteins like TAU which similarly plays a role in the maintenance of the neuronal cytoskeleton.