Abcam, ab250386, Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal PCQAP / MED15 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR13309,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250386 is the carrier-free version of
ab181158
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PCQAP also known as MED15 is a subunit of the Mediator complex an essential coactivator for transcription. MED15 helps facilitate the transcription process in eukaryotic cells by bridging transcription factors and RNA polymerase II. Its molecular mass is approximately 100 kDa. The protein is expressed widely in various tissues suggesting it has multiple roles in cellular processes.
Biological function summary
PCQAP/MED15 is important in regulating gene expression. It forms part of the large Mediator complex which interacts with various transcription factors to initiate and control chromosomal transcription. This interaction aids in the precise regulation of gene expression necessary for cell differentiation and development. Its function is quite important in the transcription machinery within the cell nucleus.
Pathways
PCQAP/MED15 integrates into the signal transduction and gene expression pathways. It has an involvement in the Wnt signaling pathway a system essential for embryonic development and cell growth. MED15 acts in conjunction with other proteins such as β-catenin which transduces the Wnt signal to the nucleus where MED15 participates in the transcriptional regulation of target genes.
PCQAP/MED15 has connections to cancer and certain neurological disorders. Its misregulation or mutation can lead to transcriptional dysregulation contributing to the onset of cancers by affecting genes that control cell proliferation. In neurological disorders PCQAP/MED15 impacts the expression of genes involved in neural functions with possible ties to disorders like Huntington's disease. The protein interacts with p53 a well-known tumor suppressor protein highlighting its role in disease development and progression.