Abcam, ab250403, Anti-URB2 antibody [EPR13404-35] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal URB2 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR13404-35,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250403 is the carrier-free version of
ab181177
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
URB2 also known as Utp23 is a protein with an approximate mass of 42 kDa. It functions as a nuclease that is essential for the maturation of small nucleolar ribonucleoprotein (snoRNP) complexes. URB2 is found primarily in the nucleolus where it plays a role in processing and modifying ribosomal RNA (rRNA). This protein is integral for the correct assembly of ribosome subunits impacting the overall efficiency and accuracy of protein synthesis.
Biological function summary
URB2 is involved in the ribosome biogenesis process which is a fundamental cellular activity. It participates as a part of the small-subunit (SSU) processome a large ribonucleoprotein complex responsible for the early steps of processing pre-18S rRNA. Through interaction with other nucleases and RNA helicases within the SSU processome URB2 ensures the precise cleavage of rRNA facilitating the formation of functional ribosomes. This activity is directly linked to cellular growth and proliferation.
Pathways
URB2 plays a significant role in the ribosome biosynthesis pathway. It interacts closely with other proteins such as Utp21 and Utp4 both important for the assemble of the SSU processome. The efficient ribosome synthesis pathway is stringent on the function of URB2 because defects in ribosomal assembly can slow down protein production affecting cellular growth. Additionally URB2 is associated with the nucleolar surveillance pathway which monitors ribosome assembly and manages stress responses in the nucleolus.
Altered URB2 function can contribute to ribosomopathies which are disorders resulting from defects in ribosome synthesis. Such defects in URB2 have been linked to conditions like Diamond-Blackfan anemia where dysfunctional ribosomal assembly leads to inadequate red blood cell production. URB2 is also associated with Treacher Collins syndrome through interactions with the TCOF1 protein impacting craniofacial development. Understanding URB2’s role in these conditions might offer insights into novel therapeutic approaches.