Abcam, ab250434, Anti-CAND1 antibody [EPR14242(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal CAND1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14242(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IP, Flow Cyt (Intra), IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250434 is the carrier-free version of
ab181216
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CAND1 also known as cullin-associated and neddylation-dissociated 1 is a scaffolding protein with a molecular mass of approximately 130 kDa. It is found in the cytoplasm and nucleus of eukaryotic cells. CAND1 plays a critical role in regulating the activity of cullin-RING ubiquitin ligases (CRLs) which are important for protein degradation processes. By interacting with neddylated cullin proteins CAND1 modulates the assembly and disassembly of these CRL complexes therefore influencing their activity.
Biological function summary
CAND1 mediates the dynamics of CRL complexes acting as a regulator of substrate specificity and turnover. CAND1 does not function alone; it is integral to a complex that includes cullin proteins and various CRL adaptors. This interaction ensures the right substrates are targeted for ubiquitination leading to proteasomal degradation. The ability of CAND1 to bind to cullin substrates is influenced by cullin neddylation and deneddylation cycles making it essential for the proper function of the ubiquitin-proteasome system.
Pathways
Studies have shown that CAND1 is important in the ubiquitin-proteasome pathway along with the neddylation pathway. Within these pathways it primarily interacts with proteins such as cullins 1 3 and 4 which are central to CRLs. By regulating these interactions CAND1 ensures that protein turnover is controlled which is important for processes like cell cycle progression and signal transduction.
Research suggests a connection between CAND1 dysregulation and certain cancers as well as neurodegenerative disorders. In cancer aberrant regulation of CAND1 can lead to uncontrolled cell proliferation due to faulty protein degradation. In neurodegenerative diseases ineffective CAND1-mediated pathways may contribute to the accumulation of damaged proteins. Understanding CAND1's relationship with key proteins in these conditions such as p53 in cancer highlights its potential as a therapeutic target.